The miR-200 Family Targeting amh Affects the Gonadal Development of Japanese Flounder

Four members of the miR-200 family in Japanese flounder (Paralichthys olivaceus) have sex-biased expression patterns, but their target genes and how they work development gonads are rarely known. Anti-Müllerian hormone (AMH) can inhibit Muller’s duct female mammals regulate formation gametes after sexual maturity. There is no teleosts, amh gene still exists. Knockout results sex reversal from male to female. Therefore, it essential explore relationship between clarify what role plays gonads. In flounder, two binding sites for 3′UTR were found through bioinformatic prediction. Double luciferase green fluorescent protein reporter experiments demonstrated be directly targeted by miR-200a miR-200b. Moreover, miR-200b reduced site 1 rather than 2. To regulatory gonadal development, we further overexpressed primary Sertoli cells testis. With overexpression miR-200a, decreased, while other sex-related genes, dmrt1 (doublesex mab-3 related transcription factor 1) gsdf (diagonal soma driven factor), increased significantly. This result indicates that regulates differentiation targeting flounder.